In this lesson from the Great Lakes Bioenergy Research Center (GLBRC), students investigate the effect of the carbohydrate source on the process of fermentation by bakers yeast. This simple investigation can serve as an introduction to the comparison of fermentation and cellular respiration or to an in-depth study of biofuels production. The fermentation occurs in resealable plastic bags, and students measure ethanol and carbon dioxide production. Supporting materials include instructional supports include background information, videos, student data sheets, and a presentation. All lesson materials are available for download as a zipped file package. The GLBRC Education site (https://www.glbrc.org/education) contains many other units, lessons, and other resources to help student learn about biofuels research and prouction.