Bat Echolocation is one of a series of Data Point resources from HHMI Biointeractive. Data Points engage student in analyzing and interpreting data from primary literature in the biological sciences. The resources are intended to provide authentic phenomena to spark student discussion and exploration, but they can also serve as three-dimensional assessment items. In this resource, students analyze a spectrograph with corresponding video stills of bats using echolocation to locate and acquire prey. The resource comes with both student and teacher materials. Each includes background material. The primary source for this Data Point may be found at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4386384/.